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Thermo mirna mimic

Webb5 apr. 2024 · Melanoma cells transfected with miRNA mimic or inhibitor were incubated at 37°C in 5% CO 2 and then detached with 0.25% trypsin solution in HBSS (Gibco; Thermo … Webb29 sep. 2024 · microRNAs are small non-coding RNAs that regulate several genes post-transcriptionally by complementarity pairing. Since discovery, they have been reported to …

miScript miRNA Mimics - Qiagen

Webb260 nm. For siRNA, 1 µM = 13.3 ng/µL. For microRNA mimic, 1 µM=14.1 ng/µL, and microRNA hairpin inhibitor, 1 µM=18.5 ng/µL see FAQs for additional information. 7. RNA may be used immediately, or aliquoted into smaller volumes to limit the number of freeze-thaw cycles. Resuspended siRNA should be WebbFor miR-9 detection, an MiRNA Q-PCR Detection Kit (GeneCopoeia, Rockville, MD, USA) was used to conduct real-time PCR on ABI 7500 thermocycler. U6 gene was used as internal reference. The PCR steps were 95°C for 10 minutes, 40 cycles of denaturation at 95°C for 15 seconds, and annealing/elongation step at 60°C for 60 seconds. electronic steering pump https://rixtravel.com

miRNA Mimic & Inhibitor Libraries Thermo Fisher Scientific - CN

WebbA genome-wide human mirVana miRNA mimic screen carried out in 10 cancer cell lines uncovered three tumor-suppressive microRNAs which target the gene BRD4, suggesting … Webb3,5-Dimethoxy-4-methylbenzoic acid undergoes reduction in the presence of sodium in ethanol to prepare 1,4-dihydro-3,5-dimethoxy- kmethylbenzoic acid. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may WebbThis Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals. RUO – Research Use Only electronics tempe az

miR-152-3p Sensitizes Glioblastoma Cells Towards Cisplatin Via ...

Category:MicroRNA-486-3p directly targets BIK and regulates apoptosis and …

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Thermo mirna mimic

Lipofectamine™ RNAiMAX Transfection Reagent - GEO Accession …

Webb12 apr. 2024 · miR-1246 mimic transfected iHMVECs were fixed with 4% paraformaldehyde (PFA) at room temperature for 10 min. After blocking with 5% goat serum in PBS for 1 h, the cells were incubated with mouse anti-human CD144 antibody (1:400, 555661; BD Pharmingen) at 4°C overnight. WebbmiRNA mimics and inhibitors) into a wide range of eukaryotic cells. Important Guidelines ∤ RNA-Lipofectamine® RNAiMAX complexes must be made in serum-free medium such …

Thermo mirna mimic

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Webb21 dec. 2024 · miScript miRNA Mimics are chemically synthesized, double-stranded RNAs which mimic mature endogenous miRNAs after transfection into cells. miScript miRNA … WebbMir 218 Miridian Mirna Mimics, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results …

Webb29 maj 2024 · miRNA mimics (agomiR) are ds for to be identified by intracelular mechanism in maturation of microRNA (DICER) and increase its amount as mature miRNA (ss). miRNA inhibitor (antagomiR) are... WebbThe final concentration of miRNA mimics was 50 nmol/L, the final concentration of miRNA inhibitors was 100 nmol/L, and the final concentration of siRNAs was 20 nmol/L. To generate pGL3-lncRNA 1308 constructs, the sequence of lncRNA 1308 mRNA was amplified by the primers listed in Table S1.

WebbSimilarly, the cells were cotransfected with lenti-LINC00888 with or without miR-126 mimics (20 nM). Lipofectamine 3000 (Thermo Fisher Scientific) was used as a … WebbMicroRNA-486-3p directly targets BIK and regulates apoptosis and invasion in colorectal cancer cells Li Feng,1 Li Jing,1 Jing Han,1 Guiying Wang,2 Yan Liu,1 Xue Zhang,1 Yudong Wang,1 Feifei Wang,2 ... Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific) was used. miR486 ... miRNA mimics strategy. Bioinform Biol Insights. …

Webb27 feb. 2024 · 生物活性： mmu-miR-6715-5p mimics are small, chemically synthesized double-stranded RNAs that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity. 运输条件： Room temperature in continental US; may vary elsewhere.

WebbmiRNA mimics are small, chemically modified double-stranded RNAs that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA … football gear for kids amazonWebbThe miR-562 mimics, inhibitor, and negative control (NC) were purchased from GenePharma (Shanghai, P.R. China). Cells were grown on 80% confluence prior to transfection. Transfection of the miR-562 mimics or inhibitor into glioma U251 and U87 cells was performed by using Lipofectamine 2000 (Invitrogen) according to the … football gaming tableWebbLipofectamine RNAiMAX Transfection Reagent provides the highest transfection efficiencies set the widest variety of cell species available siRNA-mediated gene knockdown experiments. Lipofectamine RNAiMAX … football gaming videosWebbMicroRNAs (miRNAs) are short, highly conserved small noncoding RNA molecules naturally occurring in the genomes of plants and animals. miRNAs are 17–27 … football gear for 9 year oldsWebb14 apr. 2024 · The miRNA-326 was co-transfected with 100 ng of psicheck2-NKX3.2-fragment and 40 pmol of miRNA-326 mimics or negative control (NC) mimics (RiboBio, … football gary linekerWebb14 aug. 2016 · I am trying to inhibit one miRNA in MDA-MB-231 breast cancer cells using mirVana inhibitors (Thermo Fisher) and lipofectamine. I see efficient transfection since I … football gear for saleWebbmirVana miRNA 模拟物是经过化学修饰的双链 RNA 分子，旨在模拟内源性 微小RNA (miRNA)，由于 mRNA 隔离或降解导致靶 mRNA 翻译下调。 与天然 miRNA 一样，这些 … football gear for girls