Ntf2 mediates nuclear import of ran
Web16 nov. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2–RanGDP complex to the nuclear side of the NPCs. A direct … Web18 jan. 2002 · We also used a mutant species [Ran(T24N)], which is defective in binding to nuclear transport factor 2 (NTF2), the Ran import carrier (4, 13, 14). The rate of nuclear accumulation of FL-Ran(T24N) was <4% that of wild-type FL-Ran (Fig. 1B) and was similar to the rate of diffusion of green fluorescent protein (GFP) into the nucleus (15,16).
Ntf2 mediates nuclear import of ran
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WebNTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2–RanGDP complex to the nuclear side of the NPCs. A direct NTF2–RanGDP … Web1 jan. 2014 · Therefore, p10/NTF2-mediated nuclear import of Ran-GDP is crucial to prevent exhaustion of nuclear Ran, which would cause transport arrest. Hikeshi, a recently identified NTR that mediates heat-stress induced nuclear import of Hsp70s, is proposed to couple with the ATPase cycle of Hsp70s .
WebNTF2 has a much lower affinity for RanGTP than RanGDP, and thus travels empty back to the cytoplasm after its cargo (RanGDP) is converted to RanGTP by RCC1 inside the nucleus triggering its release. This constant import of Ran by NTF2 into the nuclear interior keeps Ran concentrated inside the nucleus. View chapter Purchase book Myogenesis Web8 mrt. 2024 · For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position.
Web7 feb. 2008 · These observations may provide an explanation how CapG nuclear import can occur when only Ran or NTF2 is added. Discussion. Nuclear accumulation of CapG in permeabilized cells does not result from passive diffusion because CapG import is inhibited when Nups are obstructed by WGA, mAb414 or importin β (45–485). Web16 nov. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2–RanGDP complex to the nuclear side of the NPCs. A direct …
Web1 dec. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2-RanGDP complex to the nuclear side of the NPCs. A direct …
WebFIG. 6. Karyopherin a (Kap60) releases karyopherin b (Kap95) from RanGTP. A, Ran that was ;40% GTP and ;60% GDP bound was immobilized on a CM5 sensor chip as described under “Experimental Procedures.” Injection of 100 mg/ml Kap95 resulted in binding of 500 RU to the Ran surface. B, Ran was immobilized as in A, and 90 RU of Kap95 were … goldthwaite first united methodist churchWebscientific article headset asus rog strix fusion 300 pretoWebis recognized in the cytoplasm by NTF2 (nuclear transport fac- tor 2), which mediates its rapid translocation into the nucleus where the Ran guanine nucleotide exchange factor RCC1 (regu- goldthwaite forecastWebPaschal BM, Gerace L (1995) Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J Cell Biol 129, 925–937 CrossRef PubMed CAS Google Scholar Paschal BM, Fritze C, Guan T, Gerace L (1997) High levels of the GTPase Ran/TC4 relieve the requirement for nuclear protein transport factor 2. headset asus tuf h3WebHere we show that NTF2 is a mediator of Ran import into the nucleus and that NTF2 is rate limiting for Ran import into nuclei of permeabilized cells. Moreover, a direct … headset asus strix 7.1 realWeband probably also mediates translocation of the NTF2– RanGDP complex to the nuclear side of the NPCs. A direct NTF2–RanGDP interaction is crucial for this process, since point mutations that disturb the RanGDP–NTF2 interaction also interfere with Ran import. The subsequent nuclear accumulation of Ran also requires GTP, but not GTP ... goldthwaite girls basketballWebNuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP. The simplicity and specialization of this system, combined with the availability of crystal structures of NTF2, RanGDP and their complex, has facilitated the investigation of the … goldthwaite genealogy