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RNA Isolation with TRIzol (Invitrogen) and Qiagen RNAeasy
WebInvitrogen™ RNase-free tips (including filter tips) of many sizes are compatible with most commonly used pipettors. We also ... samples can be resuspended in RNase-free water (with 0.1 mM EDTA) or TE buffer (10 mM Tris, 1 mM EDTA) and stored at –80°C. Using a buffer solution that contains a WebEverything must be RNase free until cDNA is obtained Dissect the tissue, and freeze in liquid nitrogen. Put the tissue in a bag (seal pack, KAPAK) and mechanically powder the tissue by working on dry ice. Isolate RNA using the TRIzol Reagent (Invitrogen, #15596-018) by adding 1ml of TRIzol Reagent per 50-100 mg of tissue, pipette several times. fast for thyroid blood test
Nuclease-Free Water - Promega
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